The R&D brand elisa kit supplied by our company is not recommended to extend the incubation time for any Quantikine ELISA. Moreover, when the experimental procedure changed, we were unable to guarantee the results of the Quantikine ELISA. R&D does “quality control of the entire kitâ€, which means that we cannot support the changed experimental steps because they are not quality controlled. Extending the incubation time, or increasing the incubation temperature to shorten the incubation time will increase the background of the experiment (aka blank or zero standard). As a result, low levels of cytokines in the sample and standard will not be detected because the increased background signal will be subtracted from the values ​​in all wells. Automotive Connector Housing,Electrical Wire Automotive Connector,Automotive Electrical Connector Housing,Automotive Connectors Housing Wenzhou Langrun Electric Co.,Ltd , https://www.langrunele.com
Thank you for your technical feedback, we will serve you with more mature technology. Welcome to order our reagents again, I wish you success!