Abstract: Fluorescence spectrophotometer has been widely used in biochemistry, biomedicine and environmental chemical industry. This paper mainly introduces the functional characteristics of fluorescence spectrophotometer, the classification and advantages and disadvantages of fluorescence spectrophotometer, and the application of fluorescence spectrophotometer. And the use and its principles, finally introduced the detailed steps used in the fluorescence spectrophotometer. 1. Fluorescence emission spectrum A certain fixed wavelength of light is selected to excite the sample, and the fluorescence emission intensity generated in the sample is recorded as a function of the emission wavelength, that is, the fluorescence emission spectrum is obtained. 2. Fluorescence excitation spectrum The fluorescence emission intensity is recorded as a function of the wavelength of the excitation light by selecting a certain fluorescence emission wavelength, that is, a fluorescence excitation spectrum is obtained. 3. Time resolution technique It can be used to distinguish and separately measure the components in the mixture that overlap in the spectrum but have a difference in lifetime. The time-resolved fluorescence measurement formula is as follows: P(t) = P0 EXP (-t /Ï„) Where P(t): fits the exponential function P0: Strength value EXP: index operator t: time value Ï„: average fluorescence lifetime Fluorescence spectroscopy has the advantages of high sensitivity, strong selectivity, low sample size, simple method, wide working curve range, and can be widely used in life science, medicine, pharmacy and pharmacology, organic and inorganic chemistry. The development of the fluorescence spectrophotometer has experienced a hand-controlled fluorescence spectrophotometer, an automatic recording fluorescence spectrophotometer, and a computer-controlled fluorescence spectrophotometer. The spectrophotometer has three stages; the fluorescence spectrophotometer can be divided into two series: single beam type fluorescence spectrophotometer and double beam type fluorescence spectrophotometer. Others include the low-temperature laser Sh p ol'skill fluorescence spectrophotometer, and a fluorescence spectrophotometer with lifetime and phase discrimination. It has high sensitivity and can be used to scan the fluorescence spectrum emitted by liquid phase fluorescent markers. It can not only perform general quantitative analysis, but also infer the conformational changes of molecules in various environments, thus elucidating the relationship between molecular structure and function. . 1. The intensity of fluorescence is not high, so it is sometimes not ideal when it is linear; 2. Some reactions have a short duration of fluorescence; 3. The direction of fluorescence divergence is not concentrated, not concentrated like transmitted light, and the intensity is high; 4. Due to the interference of certain ions, the fluorescence will be quenched and the test speed must be fast. Fluorescence spectrophotometer mainstream products F96 series, F97 series fluorescence spectrophotometer and top F98 fluorescence spectrophotometer produced by Shanghai Guangguang; F-380, F-320 and F-280 fluorescence spectrophotometers produced by Tianjin Gangdong; WFY-28 fluorescence spectrophotometer produced by Tianjin Tuopu; 970CRT type fluorescence spectrophotometer produced by Shanghai Sanke; Laptops,windows Laptops,win11 Laptops,win10 Laptops Jingjiang Gisen Technology Co.,Ltd , https://www.gisentech.com
